Antiplasmodial Activity of Seed Oil of Moringa oleifera (Lam.)

Main Article Content

Dada, Ebenezer Oluyemi
Abdulahi, Sikiru Kayode

Abstract

The study was carried out to determine the antiplasmodial activity of seed oil of Moringa oleifera extracted using n-hexane solvent.

Acute toxicity test was carried out on twelve albino mice grouped into 3 according to seed oil concentrations (250, 500 and 1000 mg/kg respectively) with each group having four mice. Thirty-five mice were infected with Plasmodium berghei and the mice were randomized into five groups of seven mice each (groups A, B, C, D and E) for antiplasmodial activity. Group A - negative control (non-treated), group B - positive control (chloroquine treated); group C (800 mg/kg), group D (400 mg/kg) and group E (200 mg/kg) of seed oil of Moringa oleifera. All groups were left untreated until after five days when 0.2 mL treatment dose for each group was administered. Treatment was carried out in four days and left for another five days for post treatment effect.

The acute toxicity test showed that the seed oil of Moringa oleifera was safe and nontoxic to all mice. There was daily gradual reduction in PCV values; however group with the highest suppression of parasitemia had the highest PCV value after treatment. Group A as expected had the lowest PCV value of 22.23±1.98% which fell short of normal range (40 - 55%) and had its parasitemia load increased by 205% while in other groups, B, C, D and E the parasitemia had decreased by 100%, 97.02%, 90.48% and 67.65%respectively after treatment.

Overall, the seed oil of M. oleifera at high concentrations showed a competitive parasite inhibition activity when compared with the result obtained in positive control group; however, few deaths recorded during and after treatment called for further investigation to determine its suitability for the treatment of malaria.

Keywords:
Antiplasmodial, Moringa oleifera, seed oil

Article Details

How to Cite
Oluyemi, D. E., & Kayode, A. S. (2021). Antiplasmodial Activity of Seed Oil of Moringa oleifera (Lam.). Journal of Advances in Medical and Pharmaceutical Sciences, 23(1), 24-32. https://doi.org/10.9734/jamps/2021/v23i130214
Section
Original Research Article

References

Muhammed D, Dada EO, Muazu M, Jumbo EI, Uzokwe VI. Antiplasmodial activity of ethanolic leaf extract of Eucalyptus citriodora in swiss albino mice infected with Plasmodium berghei NK 65 South Asian Journal of Research in Microbiology. 2018;2(2):1-10.

Muhammed D, Dada EO, Iyaji FO, Abraham OJ, Chijioke NA. Investigation of biochemical parameters of Plasmodium berghei infected mice after administration of ethanolic leaf extract of Eucalyptus citriodora. International Journal of Pathogen Research. 2019;2(2):1-9.

Ogundolie OO, Dada EO, Osho IB, Oloruntola DA. Effects of raw ethanolic seed extract of Tetracarpidium conophorum on heamatological and histopathological parameters in Swiss albino mice infected with Plasmodium berghei. Journal of Applied Life Sciences International. 2017;12(2):1-14.

Dada EO,Muhammed D. Effect of ethanolic leaf extract of Eucalyptus citriodora Hook on haematological parameters of swiss albino mice infected with Plasmodium berghei NK 65. South Asian Journal of Parasitology. 2018; 1(2):1-8.

Ekeopara CA, Ugoha AM. the contributions of african traditional medicine to Nigeria’s health care delivery system. Journal of Humanities and Social Science. 2017; 22(5):32-43.

Boukandoul S, Casal S, Zaidi F. The potential of some Moringa species for seed oil production. Agriculture. 2018;8(150): 1-13.

Kayode RM, Afolayan AJ. Cytotoxicity and Effect Of Extraction Methods On The Chemical Composition Of Essential Oils of Moringa oleifera seeds. J Zhejiang Univ-Sci B (Biomed & Biotechnol). 2015;16(8): 680-689.

Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidant. 2017;6(91): 1-13.

Efeovbokhan VE, Hymore FK, Raji D, Sanni SE. Alternative solvents for Moringaoleifera seeds extraction. Journal of Applied Sciences. 2015;15(8): 1073-82.

Olaniran O, Adetuyi FC, Omoya FO, Odediran SA, Hassan-olajokun RE, Awoyeni EA, Odetoyin BW, Adesina A, Awe A, Bejide RA, Odujoko O, Akinyemi LO, Oyetoke OO, Afolayan DO. Antiplasmodial, antipyretic, haematological and histological effects of the leaf extracts of Moringa oleifera in Plasmodium berghei infected mice. Journal of Advances in Medicine and Medical Research. 2019; 29(4):1-13.

Alo AA, Dada EO, Muhammed D. Phytochemical Screening and antiplasmodial activity of ethanolic bark extract of Khaya grandifoliola in swiss albino mice infected with Plasmodium berghei NK65.South Asian Journal of Parasitology. 2018;1(4):1-8.

Da O, Yerbanga RS, Traore/Coulibaly M, Koama BK, Kabre Z, Tamboura S, Dakuyo ZP, Sekhoacha MP, Matsabisa MG, Nikiema JB, Ouedraogo JB, Ouedraogo GA. Evaluation of the antiplasmodial activity and lethality of the leaf extract of Cassia alata L. (Fabaceae). Pakistan Journal of Biological Sciences. 2016; 19(4):171-178.

Zade V, Dabhadkar D, Thakare V, Pare S. Evaluation of potential aphrodisiac activity of Moringa oleifera seed In male albino rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2013;5(4):683-689.

Orman E, Addo P, Ofori MF, Adosraku RK. Investigating the In-vivo antiplasmodial properties of aqueous extract of Moringa oleifera Lam. (Moringaceae) Leaves. British Journal of Pharmaceutical Research. 2015;5(6):419-430.

Olasehinde GI, Ayanda OI, Ajayi AA, Nwabueze AP. In vivo antiplasmodial activity of crude n-hexane and ethanolic extracts of Moringa oleifera (Lam.) Seeds on Plasmodium berghei. International Journal of Medicinal Plant Research. 2012;1(5):50-54.

Daskum AM, Godly C, Qadeer MA. Antiplasmodial activities of crude Moringa oleifera leaves extracts on chloroquine sensitive Plasmodium falciparum (3D7). Bayero Journal of Pure and Applied Sciences. 2019;12(1):315-320.

Osman HM, Shayoub ME, Babiker EM, Faiza AO, Ahmed MM, Osman B, Elhassan AM, Taha KK. Assessment of acute toxicity and LD50 of Moringa oleifera ethanolic leave extract in albino rats and rabbits. Journal of Medical and Biological Science Research. 2015;1(4): 38-43.