Antimicrobial Efficacy of Selected Natural Products on Microorganisms Isolated from Throat of Patients with Throat Infection
Journal of Advances in Medical and Pharmaceutical Sciences,
Introduction: Natural products have been used in traditional medicines for treatment of infections due to the antimicrobial activity they exhibit. This study therefore evaluates the efficacy of honey, ginger (Zingiber officinale) and garlic (Allium sativum) extracts on microorganisms isolated from throat of patients with throat infection.
Methods: The antibacterial and antifungal efficacy of honey, ginger (Zingiber officinale) and garlic (Allium sativum) extracts was investigated against microorganisms isolated from throats of infected patients at the ENT Department of State Specialist Hospital, Akure, using agar disc diffusion and agar well diffusion technique respectively.
Results: Bacteria isolated from patients with throat infection were Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, and Proteus mirabilis while the fungal isolates were Candida albicans and Candida tropicalis. The antibacterial and antifungal assay results showed that all bacterial isolates were inhibited by honey, garlic and ginger extract. Honey, ginger and garlic showed highest inhibition against P. mirabilis (19.01±0.31 mm), P. aeruginosa (20.20±0.42 mm) and S. aureus (23.00±0.01 mm) respectively also, antifungal assay results showed that all the extracts had antifungal effect on the fungal isolates. The combination of equal concentrations of honey plus garlic showed the highest inhibitory effect on all the test bacteria followed by honey plus ginger then garlic plus ginger while the combination of honey plus garlic had the highest inhibitory effect on Candida albicans (21.63±0.02 mm) but garlic plus ginger combination showed the highest inhibitory effect on Candida tropicalis (21.68±0.04 mm).
Conclusion: The result of this study therefore showed that the bacteria and fungi isolated from throat of patients with throats infection demonstrated sensitivity towards the tested samples of honey, garlic and ginger and hence, can serve as effective therapeutic agents in the treatment of throat infections.
- Antibacterial activity
- antifungal activity
- natural products
- throat infection
How to Cite
Tala SD, Gatsing D, Fodouop SPC, Fokunang C, Kengni F, Djimeli MN. In vivo anti-salmonella activity of aqueous extract of Euphorbia prostrata Aiton (Euphorbiaceae) and its toxicological evaluation. Asian Pacific Journal of Tropical Biomedicine. 2015;5(4):310-318.
Zakia M, Nihal A, Reem ElB. A natural alternative to conventional antibiotics. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2014;5(4):588-599.
Nanda MS, Mittal SP, Gupta V. Role of honey as adjuvant therapy in patients with sore throat. National Journal of Physiology, Pharmacy and Pharmacology. 2016;7(4): 412-415.
Yahaya O, Yabefa JA, Umar IO, Datshen MM, Egbunu ZK, Ameh J. Combine antimicrobial effect of ginger and honey on some human pathogens. British Journal of Pharmacology and Toxicology. 2012;3(5): 237-239.
Metwali Z, Abdalla N, El Barrani R. A natural alternative to conventional antibiotics. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2014;5(4):588-599.
Mshelia BM, Adeshina GO, Onaolapo JA. The antibacterial activity of honey and lemon juice against Streptococcus pneumoniae and Streptococcus pyogenes isolates from respiratory tract infections. Advances in Biotechnology and Microbiology. 2017;4(5):001-008.
Vallianou NG, Gounari P, Skourtis A. Honey and its anti-inflammatory, anti-bacterial and anti-oxidant properties. General Medicine. 2014;2:132.
Del Mar CB, Glasziou PP, Spinks AB. Antibiotics for sore throat. Cochrane Database Syst Rev. 2006;4:CD000023.
Cheesbrough M. District laboratory practice in tropical countries. Part 2, UK; Cambridge University Press; 2006.
Ogodo C, Ekeleme UG. In-vitro antibacterial activity of garlic cloves and ginger rhizomes on food-borne pathogens. International Journal of Basic and Applied Sciences. 2013;2(4):387-392.
Arekemase MO, Kayode RMO, Ajiboye AE. Antimicrobial activity and phyto-chemical analysis of Jatropha curcas plant against some selected microorganisms. International Journal of Biology. 2011;3(3): 52-59.
Amel AS, Fadwa ME, Smah AS, Nazar AO. Antimicrobial activity of Zingiber officinale (Ginger) oil against bacteria isolated from children throat. Journal of Microbiology and Biomedical Research. 2015;3:14-21.
Clinical and Laboratory Standard Institute, CLSI. Performance standards for antimicrobial susceptibility testing. Twenty-fourth Informational Supplement. CLSI Document M 100-S24. Wayne, P. A: 550-84; 2014.
Owoseni AA, Ajayi A. Antimicrobial properties of ethanolic and aqueous extracts of Cymbopogon citratus on selected bacteria and fungi. Journal of Medicine and Applied Bioscience. 2010;2(4):64–73.
Belstrom D, Holmstrum P, Nielsen CH, Kirbkby N, Twetman S. Bacterial profiles of saliva in relation to diet, lifestyle factors and socioeconomic status. Journal of Oral Microbiology. 2014;6(1):23609.
Kenealy T. Acute infective sore throat. Clinical Evidence Handbook. 2015;91(10): 526-527.
Ahmad MM, Kurawa ZM, Shuaibu I, Yahaya G. Microbiological assessment of bacterial isolates from ear, nose and throat (ENT) among patients attending Aminu Kano teaching hospital. Nigerian Journal of Basic and Applied Science. 2016;24(1):15-18.
Bansal V, Medhi B, Pandhi P. Honey – A remedy rediscovered and its therapeutic utility. Kathmandu University Medical Journal (KUMJ). 2005;3(3):305-9.
Olaitan PB, Adeleke OE, Ola IO. Honey: A reservoir for microorganisms and an inhibitory agent for microbes. Afr Health Sci. 2007;7(2):159-65.
Kamaruddin MY, Zainabe SA, Anwar S, Razif MA, Yassim MY. The efficacy of honey dressing on chronic wounds and ulcers. In: Juraj M, Editor. Honey: Current Research and Clinical Uses. New York: Nova Science Publishers. 2012;185-96.
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