https://www.journaljamps.com/index.php/JAMPS <p style="text-align: justify;"><strong>Journal of Advances in Medical and Pharmaceutical Sciences (ISSN:&nbsp;2394-1111)</strong>&nbsp;aims to publish high quality papers (<a href="/index.php/JAMPS/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of&nbsp;Medical and Pharmaceutical Sciences.&nbsp;By not excluding papers based on novelty, this journal facilitates the research and wishes to publish papers as long as they are technically correct and scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer-reviewed, open-access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Journal of Advances in Medical and Pharmaceutical Sciences 2394-1111 https://www.journaljamps.com/index.php/JAMPS/article/view/863 <p><strong>Background and Aim:</strong> Light at night (LAN) and methamphetamine (Meth) are emerging environmental and pharmacological stressors implicated in neuroendocrine and reproductive dysfunction. This study investigated the independent and combined effects of LAN and Meth on reproductive hormone profiles, cortisol levels, estrous cyclicity and histological integrity of the ovaries and uterus in female Albino Wistar rats.</p> <p><strong>Methodology: </strong>Twenty-four adult female Albino Wistar rats (120–150 g) were randomly assigned into four groups of six (n = 6). Group A (control) received normal saline under a standard 12:12-hour light-dark cycle. Group B (LAN) was exposed to 24-hour continuous light for 28 days. Group C (Meth) received methamphetamine at 5mg/kgbw orally for 28 days under standard light-dark conditions. Group D (LAN + Meth) received both exposures simultaneously. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), estrogen, progesterone, and cortisol were measured using ELISA. Estrous cycles were monitored through vaginal smear cytology. Ovarian and uterine tissues were weighed and examined histologically. Data were analyzed using one-way ANOVA with post hoc LSD at p ≤ 0.05.</p> <p><strong>Results: </strong>Significant reductions in LH were observed in all treated groups compared to control (F= 8.039, p ≤ 0.05). FSH levels decreased significantly in Groups B and C, while Group D showed a significant increase compared to Group C (p ≤ 0.05). Estrogen levels were significantly elevated in the LAN group, whereas progesterone changes were not significant. Serum cortisol was significantly suppressed in all treated groups, with the greatest reduction in Meth-exposed rats. Estrous cycles showed progressive irregularity in all treated groups. Histology revealed mild degenerative and inflammatory changes, most notable in the LAN group, with largely preserved architecture overall.</p> <p><strong>Conclusion: </strong>LAN and Meth disrupt hypothalamic–pituitary–gonadal axis function and stress hormone regulation, with LAN producing more pronounced endocrine and histological alterations.</p> Makuachukwu Francisca Chinweokwu Mgbemena Ed. Nwobodo Copyright (c) 2026 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2026-05-30 2026-05-30 28 6 1 12 10.9734/jamps/2026/v28i6863 https://www.journaljamps.com/index.php/JAMPS/article/view/864 <p>Natural products remain a cornerstone of drug discovery, providing structurally diverse bioactive compounds with significant therapeutic potential. Their incorporation into nanomedicine, either as active pharmaceutical ingredients or functional excipients, has expanded rapidly due to their ability to improve solubility, bioavailability, and targeted delivery. However, a fundamental assumption persists in current research: phytochemicals are typically treated as discrete, chemically uniform, and molecularly dispersed prior to nanoparticle formulation. This reductionist view overlooks the inherent complexity of natural extracts, which exist as multicomponent and dynamically interacting systems.</p> <p>In this work, we propose a hypothesis that challenges this assumption by introducing the concept of pre-nanoparticle molecular organization (PNMO). We suggest that phytochemicals in crude or semi-purified extracts undergo intrinsic self-aggregation and supramolecular assembly before nanoparticle fabrication. These assemblies are governed by non-covalent interactions, including hydrogen bonding, π–π stacking, hydrophobic forces, and electrostatic interactions, resulting in pre-formed molecular clusters.</p> <p>We argue that PNMO critically influences key nanoparticle characteristics such as size distribution, morphology, colloidal stability, and drug loading efficiency, as well as downstream biological performance. Neglecting this pre-formulation state may lead to inconsistencies in physicochemical characterization, poor reproducibility, and challenges in quality control. Moreover, PNMO may alter biodistribution, cellular uptake, and therapeutic outcomes, complicating structure–activity relationship analyses.</p> <p>By integrating principles from supramolecular chemistry and nanomedicine, this review highlights PNMO as an essential yet overlooked design parameter. Its systematic characterization and control may enable more reproducible formulations and facilitate the rational development and clinical translation of phytochemical-based nanotherapeutics.</p> Salaheldin Elabiad Nazmi Özer Copyright (c) 2026 Author(s). The licensee is the journal publisher. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2026-05-30 2026-05-30 28 6 13 26 10.9734/jamps/2026/v28i6864